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| Antigenic Specificity | 6X His Tag |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | n/a |
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| Isotype | n/a |
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| Format | DyLight? 549 conjugate |
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| Size | 100 μg |
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| Concentration | 1.0 mg/mL |
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| Applications | FLISA, IF Microscopy, Western Blot. Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins containing the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. The emission spectra for this DyLight? conjugate match the principle output wavelengths of most common fluorescence instrumentation. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This affinity purified antibody is directed against the 6X HIS epitope tag and is useful in determining its presence in over expressed proteins in various assays. The antibody recognizes the 6X HIS epitope tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
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| Immunogen | This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the 6X HIS epitope tag (H-H-H-H-H-H) conjugated to KLH using maleimide. |
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| Other Names | rabbit anti-6X HIS EPITOPE TAG Antibody DyLight?549 conjugation, DyLight?549 conjugated rabbit anti-6X HIS EPITOPE TAG Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody |
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| Gene, Accession # | n/a |
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| Catalog # | 600-442-382 |
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| Price | |
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| Order / More Info | 6X His Tag Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Yi CE, Ba L, Zhang L, Ho DD, Chen Z. (2005) Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain. J Virol.Sep;79(18):11638-46. |
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