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| Antigenic Specificity | 6X His Tag |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | n/a |
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| Isotype | n/a |
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| Format | affinity purified |
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| Size | 25 μL |
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| Concentration | 0.95 mg/mL |
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| Applications | ELISA, Immunohistochemistry, Western Blot. Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope. The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins. This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins. Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry. |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag. To date, this antibody has reacted with all His tagged proteins so far tested. In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins. The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells. |
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| Immunogen | This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide. |
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| Other Names | rabbit anti-6X HIS EPITOPE TAG Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody |
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| Gene, Accession # | n/a |
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| Catalog # | 600-401-382S |
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| Price | |
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| Order / More Info | 6X His Tag Antibody from ROCKLAND IMMUNOCHEMICALS INC. |
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| Product Specific References | Yi CE, Ba L, Zhang L, Ho DD, Chen Z. (2005) Single amino acid substitutions in the severe acute respiratory syndrome coronavirus spike glycoprotein determine viral entry and immunogenicity of a major neutralizing domain. J Virol.Sep;79(18):11638-46.Lecona E, Rodriguez-Acebes S, Specks J, Lopez-Contreras AJ, Ruppen I, Murga M, Munoz J, Mendez J, Fernandez-Capetillo O. (2016) USP7 is a SUMO deubiquitinase essential for DNA replication. Nat Struct Mol Biol. 2016 Apr;23(4):270-7. doi: 10.1038/nsmb.3185. Epub 2016 Mar 7.Niederkofler V, Baeriswyl T, Ott R, Stoeckli ET. (2010) Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance. Development. 2010 Feb;137(3):427-35. doi: 10.1242/dev.042515. Epub 2010 Jan 7. |
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