| Description | The Hydrogen Peroxide Detection Kit is designed to quantitatively measure H2O2 in a variety of samples. A hydrogen peroxide standard is provided to generate a standard curve for the assay and all samples should be read off the standard curve. Samples are mixed with the Colorimetric Substrate and the reaction initiated by addition of horseradish peroxidase. The reaction is incubated at room temperature for 15 minutes. The HRP reacts with the substrate in the presence of hydrogen peroxide to convert the colorless substrate into a colored product. The pink product is read at 560 nm. Increasing levels of H2O2 cause a linear increase in color. Direct Quantitative Assay. Intra Assay Precision: Three buffer samples were run in replicates of 20 in an assay. The mean and precision of the calculated concentrations were: Sample 1- 82.2 μM, 2.1% CV Sample 2- 53.1 μM, 2.4% CV Sample 3- 19.4 μM, 5.9% CV Inter Assay Precision: Three buffer samples were run in duplicate in twelve assays run over multiple days by three operators. The mean and precision of the calculated concentrations were: Sample 1- 79.9 μM, 3.7% CV Sample 2- 49.5 μM, 4.5% CV Sample 3- 18.4 μM, 4.3% CV. Range: 3.125 - 100 μM. Sensitivity: 1.83 μM. Time: 15 minutes. |
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