| Description | The Prostaglandin E2 (PGE2) EIA kit is designed to quantitatively measure PGE2 present in serum, plasma, urine, saliva, cells, tissue, and tissue culture media samples. This EIA kit allows for the widest variations in sample size, sensitivity and assay timing of any PGE2 kit. The protocol variations are outlined below: The Regular Format, which uses 50 μL of sample or standard, is shown on pages 10-11, gives results in 2.5 hours. The Low Sample Volume Format, which uses 25 μL of sample or standard, is outlined on page 12-13, also gives results in 2.5 hours, but uses lower sample volumes. The High Sensitivity Format, which uses 100 μL of sample or standard, is shown on page 14-15, also gives results in 2.5 hours, but is the highest sensitivity kit of any type available. The Overnight Format, which uses 50 μL of sample or standard, is shown on page 16-17, and uses a primary overnight incubation at 4°C to fit your work flow. This format has similar sensitivity to the Regular Format outlined above. Sandwich EIA (Enzyme Immunoassay). Intra Assay Precision: Three human samples were diluted with Assay Buffer and run in replicates of 20 in an assay. The mean and precision of the calculated Prostaglandin E2 concentrations were: Sample 1- 11.7 pg/mL, 12.3% CV Sample 2- 98.6 pg/mL, 6.3% CV Sample 3- 131.2 pg/mL, 4.9% CV Inter Assay Precision: Three human samples were diluted with Assay Buffer and run in duplicates in seventeen assays run over multiple days by four operators. The mean and precision of the calculated Prostaglandin E2 concentrations were: Sample 1- 12.3 pg/mL, 8.8% CV Sample 2- 100.5 pg/mL, 8.1% CV Sample 3- 134.7 pg/mL, 9.8% CV. Range: HS: 1.953 - 500 pg/ml. LV: 15.625 - 1000 pg/ml. RG: 3.906 - 500 pg/ml. ON: 3.906 - 500 pg/ml. Sensitivity: 3.07 pg/ml. |
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