| 產(chǎn)品詳情 |
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| Antigenic Specificity | ACTB (N-terminal arginylated ) |
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| Clone | polyclonal |
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| Host Species | Rabbit |
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| Reactive Species | human, rat, mouse |
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| Isotype | n/a |
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| Format | unconjugated |
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| Size | n/a |
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| Concentration | n/a |
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| Applications | WB, Dot, ICC |
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| Reviews / Ratings | If you have used this antibody, please help fellow researchers by submitting reviews to pAbmAbs and antYbuddY. |
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| Description | Rabbit Anti-Human ACTB polyclonal antibody for WB, Dot, ICC. Actin, cytoplasmic 1 (UniProt P60709; also known as beta-actin, PS1TP5-binding protein 1) is encoded by the ACTB (also known as BRWS1, PS1TP5BP1) gene (Gene ID 60) in human. Post-translational protein arginylation is mediated by Arg-transfer RNA (-tRNA) protein transferase (Ate1; arginyltransferase). β- and β-actins are the major structural components of the actin cytoskeleton in nonmuscle cells. Despite their similarity, the β-, but not the β-, actin is N-terminally arginylated in vivo, where the first two N-terminal amino acids are removed and replaced with an arginine (from NH2-MDDDIAAL- to NH2-RDDIAAL-). In the absence of arginylation, actin forms aggregates during cell-free polymerization. Fibroblasts derived from Ate1 knockout (KO) mice show collapsed lamella, which can be rescued by reintroducing N-terminally arginylated β-actin into the Ate1 KO cells. Intracellular actin polymer levels are shown to drastically decrease in arginylation-deficient cells, which results from changes in actin polymerization properties. |
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| Immunogen | C-terminally KLH-conjugated linear peptide corresponding to human beta-actin N-terminal sequence with arginylation modification. |
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| Other Names | n/a |
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| Gene, Accession # | Gene ID: 60, UniProt: P60709 |
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| Catalog # | CABT-B1633 |
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| Price | |
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| Order / More Info | ACTB (N-terminal arginylated ) Antibody from CREATIVE DIAGNOSTICS |
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| Product Specific References | n/a |
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