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        產品名稱 PE Annexin V Apoptosis Detection Kit with 7-AAD from BIOLEGEND
        產品貨號 640934
        產品價格 現(xiàn)貨詢價,電話:010-67529703
        產品規(guī)格 100 tests
        產品品牌 BIOLEGEND
        產品概述
        產品詳情
        Edit&nbsp
        Product NamePE Annexin V Apoptosis Detection Kit with 7-AAD
        DescriptionBioLegend's PE Annexin V Apoptosis Detection Kit with 7-AAD has been specifically designed for the identification of apoptotic and necrotic cells.Annexin V (or Annexin A5) is a member of the annexin family of intracellular proteins that binds to phosphatidylserine (PS) in a calcium-dependent manner. PS is normally only found on the intracellular leaflet of the plasma membrane in healthy cells, but during early apoptosis, membrane asymmetry is lost and PS translocates to the external leaflet. Fluorochrome-labeled Annexin V can then be used to specifically target and identify apoptotic cells. Annexin V Binding Buffer is recommended for use with Annexin V staining. Annexin V binding alone cannot differentiate between apoptotic and necrotic cells. To help distinguish between the necrotic and apoptotic cells we recommend use of our 7-amino-actinomycin D (7-AAD) solution. Early apoptotic cells will exclude 7-AAD, while late stage apoptotic cells will stain positively, due to the passage of these dyes into the nucleus where they bind to DNA.7-AAD (7-amino-actinomycin D) has a high DNA binding constant and is efficiently excluded by intact cells. It is useful for DNA analysis and dead cell discrimination during flow cytometric analysis. When excited by 488 laser light, 7-AAD fluorescence is detected in the far red range of the spectrum (650 nm long-pass filter).
        Size100 tests
        ConcentrationFlow Cytometry (FC)
        Applicationsn/a
        Other NamesKit includes: PE Annexin V, 7-AAD solution, and Binding Buffer
        Gene, Accession, CAS #Gene ID: 308
        Catalog #640934
        Price
        Order / More InfoPE Annexin V Apoptosis Detection Kit with 7-AAD from BIOLEGEND
        Product Specific ReferencesPubMed: 24560783
        產品資料
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