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產(chǎn)品名稱

functional IGFBP-3 LIA from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號

E04A

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

1 x 96 well

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	functional IGFBP-3 LIA
Description	The Eagle Biosciences IGFBP-3 LIA Assay Kit utilizes specific and high affinity antibodies for IGFBP-3, the antibodies are immobilized on the microtitre plate. The ligand, biotinylated IGF-I is pre-dispensed in excess into the needed wells. The sample is diluted outside within a special dilution buffer (Sample Buffer PP); all naturally bound IGFs are thereby released from their binding proteins. By adding an aliquot of such diluted sample with free IGFBP-3 to the ligand containing wells, the biotinylated IGF-I occupies all existing specific binding sites of the IGFBP-3. All IGFBP-3 molecules are bound afterwards to the microtitre plate, however by using a Strepavidin-Peroxydase-(POD)-Conjugate only the complexes of IGFBP-3/biotinylated IGF-I are involved in signal generation (in the closing POD-substrate reaction), thus, only functional IGFBP-3 is being quantified. Sample Types: serum, plasma. Range: 0.4 - 30 ng/mL. Sensitivity: 0.4 ng/mL. Time: 3.5 hours ? All currently existing IGFBP-3 immunoassays use the binding of specific anti-IGFBP-3 antibodies for signal generation and thus IGFBP-3 quantification. The failure of differentiation between complete IGFBP-3 molecules and their respective fragments (derived physiologically due to the different proteases activities) is unavoidable in this system. Because one molecule IGFBP-3 can be cleaved in several fragments often false high quantitative values are measured. Based on this methodology it is not possible to differentiate between high IGFBP-3 levels in fact, or, a high degree of fragmentation. The incidental attempts to use monoclonal antibodies with a binding region represented only by the intact IGFBP-3 molecule are indirect, imprecise and insufficient. The activities of all effective proteases, which have different sites of action and therefore generate different kind of fragments, are disregarded.
Size	1 x 96 well
Concentration	n/a
Applications	RUO
Other Names	IGFBP-3, Insulin Growth Factor Binding Protein
Gene, Accession, CAS #	n/a
Catalog #	E04A
Price
Order / More Info	functional IGFBP-3 LIA from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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