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產(chǎn)品名稱

Hydroxypyridinium Crosslinks HPLC Assay (100 Tests) from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號

CSL34-H100

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

100 tests

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Hydroxypyridinium Crosslinks HPLC Assay (100 Tests)
Description	For the determination of the pyridinium crosslinks, the samples are hydrolyzed and extracted on solid phase extraction cartridges. The probe is centrifuged and 100 μl of the supernatant are injected into the HPLC system. The isocratic separation is via HPLC at 30°C using a ``reversed phase'' column. One run lasts 15 minutes. The chromatograms are recorded by a fluorescence detector. The quantification is performed with the delivered plasma calibrator; the concentration is calculated via integration of the peak areas or heights. Sample Types: urine. Time: 4 hours 20 minutes ? Pyridinium and deoxypyridinium are built as intramolecular crosslinkers during the maturation of collagen. During bone resorption collagen is degraded and the pyridinium-crosslinks are excreted. They are not reutilized by the body and excreted in the urine. The crosslinks are not released during the de novo synthesis of collagen. They are specific for resorptive processes. Neither PYD nor DPD are taken up by nutrition. Therefore the determination of the crosslinks is independent from the diet. PYD is found in cartilage, ligaments and bone, whereas DPD is rather specific for bone. The crosslink excretion shows a strong circadian rhythm with a maximum in the early morning hours. Reliable data can be obtained with a 24h or a creatinine corrected morning urine. An elevated crosslink excretion is shown in osteoporotic women. Crosslink levels decrease during treatment with estrogen (Seibel et al. 1994). Therefore they are suited for therapy monitoring (Ganero et al. 1994). The measurement of pyridinium crosslinks is an independent predictor for future bone fractures (Ganero et al. 1995). Increased crosslink concentrations are also described in patients with Paget's Disease, primary hyperparathyroidism and bone metastasis.
Size	100 tests
Concentration	n/a
Applications	RUO
Other Names	Hydroxypyridinium Crosslinks, OH PYD, PYD, DPD, Pyridinium, deoxypyridinium
Gene, Accession, CAS #	n/a
Catalog #	CSL34-H100
Price
Order / More Info	Hydroxypyridinium Crosslinks HPLC Assay (100 Tests) from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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