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產(chǎn)品名稱

FRET No Wash cAMP Assay Kit from AAT BIOQUEST, INC.

產(chǎn)品貨號(hào)

36380

產(chǎn)品價(jià)格

現(xiàn)貨詢價(jià)，電話：010-67529703

產(chǎn)品規(guī)格

10 plates

產(chǎn)品品牌

AAT BIOQUEST, INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	FRET No Wash cAMP Assay Kit
Description	Screen Quest? FRET No Wash cAMP Assay Kit. Screen Quest? FRET No Wash cAMP Assay Kit provides a convenient assay method for monitoring the activation of adenylyl cyclase in G-protein coupled receptor systems. Compared to other commercial ELISA cAMP assay kits, this homogenous cAMP assay kit does not require a wash step or the acetylation step. The assay is based on the competition for a fixed number of anti-cAMP antibody binding sites between the fluorescent cAMP tracer and non-labeled free cAMP. Free cAMP displaces the fluorescent cAMP tracer from the HRP-cAMP/anti-cAMP antibody complex. The anti-cAMP antibody is labeled with our TR Fluor? Eu while the cAMP tracer contains our trFluor? 650. In the absence of cAMP, trFluor? 650-cAMP conjugate is bound to TR Fluor? Eu-labeled anti-cAMP antibody exclusively to have a strong FRET. However, the unlabeled free cAMP in the test sample competes for the TR Fluor? Eu-labeled anti-cAMP antibody conjugate, therefore inhibits the binding of trFluor? 650-cAMP to anti-cAMP antibody. The trFluor? 650 labeled cAMP tracer only has fluorescence lifetime of nanosecond while TR Fluor? Eu-labeled anti-cAMP antibody-bound fluorescent cAMP tracer has much longer fluorescence lifetime value due to the TR-FRET. The magnitude of FRET is proportional to the concentration of cAMP in a sample. The assay can be performed in a convenient 96-well or 384-well microtiter-plate format.
Size	10 plates
Concentration	n/a
Applications	Biossay, Fluorometric
Other Names	n/a
Gene, Accession, CAS #	n/a
Catalog #	36380
Price
Order / More Info	FRET No Wash cAMP Assay Kit from AAT BIOQUEST, INC.
Product Specific References	n/a
產(chǎn)品資料

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