| Description | Purity > 90% as determined by SDS-PAGE. MW: 44.1kDa. Full Length: 1-216aa. N-terminal 10xHis-SUMO-tagged and C-terminal Myc-tagged. Escherichia coli (strain K12). Dioxygenase that repairs alkylated DNA and RNA containing 3-methylcytosine or 1-methyladenine by oxidative demethylation. Has highest activity towards 3-methylcytosine. Has lower activity towards alkylated DNA containing ethenoadenine, and no detectable activity towards 1-methylguanine or 3-methylthymine. Accepts double-stranded and single-stranded substrates. Requires molecular oxygen, alpha-ketoglutarate and iron. Provides extensive resistance to alkylating agents such as MMS and DMS (SN2 agents), but not to MMNG and MNU (SN1 agents). |
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