| 產(chǎn)品詳情 |
| Edit |   |
| Product Name | Rat TNF-α ELISA KIT |
| Description | The Eagle Biosciences Rat TNF Alpha (TNF-α) ELISA Assay Kit employs the quantitative sandwich enzyme immunoassay technique. A monoclonal antibody specific for TNF Alpha (TNF-α) has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNF Alpha (TNF-α) present is bound by the immobilized antibody. Following incubation unbound samples are removed during a wash step, and then a detection antibody specific for TNF Alpha (TNF-α) is added to the wells and binds to the combination of capture antibody-TNF-α in sample. Following a wash to remove any unbound combination, and enzyme conjugate is added to the wells. Following incubation and wash steps a substrate is added. A colored product is formed in proportion to the amount of TNF Alpha (TNF-α) present in the sample. The reaction is terminated by addition of acid and absorbance is measured at 450nm. A standard curve is prepared from seven TNF Alpha (TNF-α) standard dilutions and TNF Alpha (TNF-α) sample concentration determined. Sample Types: serum, plasma, cell culture. Range: 31.25 - 2000 pg/ml. Sensitivity: 15 pg/ml. Time: 3.5 hours ? The Eagle Biosciences Rat TNF Alpha (TNF-α) ELISA Assay Kit (enzyme-linked immunoassay kit) is intended for the quantitative determination of rat γ-interferon (TNF-α ) concentrations in cell culture supernates, serum, and plasma. The Eagle Biosciences Rat TNF Alpha (TNF-α) ELISA Assay Kit is for research use only and not to be used in diagnostic procedures. |
| Size | 1 x 96 well |
| Concentration | n/a |
| Applications | RUO |
| Other Names | Tumor Necrosis Factor alpha, TNF-Alpha, TNF-α, TNF Alpha |
| Gene, Accession, CAS # | n/a |
| Catalog # | TNA21-K01 |
| Price | |
| Order / More Info | Rat TNF-α ELISA KIT from EAGLE BIOSCIENCES INC. |
| Product Specific References | n/a |
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