| 產(chǎn)品詳情 |
| Edit |   |
| Product Name | Tris-Glycine Buffer Powder (pH 8.3) (5 L) (10 pouches) |
| Description | Medicago AB Products Are Available Worldwide. For Research Use Only. The most common running buffer in native (non-denaturing) homogeneous and gradient polyacrylamide gel electrophoresis (PAGE). Tris-Glycine also has applications in Western blotting. Tris-Glycine gels use a stacking gel to compress the sample into a narrow band before it enters the resolving gel. This leads to much sharper bands than would be seen in gels lacking a stacking gel. Tris-Glycine gels resolve proteins by charge/size. However, very small proteins and peptides do not resolve well due to interference from the glycine/pH discontinuity front. TG buffer is used to make a Tris-glycine/20% methanol Western transfer buffer, which is the most common protein transfer buffer for wet blot transfers. The methanol prevents the gel from swelling during transfer and enhances protein binding to nitrocellulose. Make sufficient transfer buffer to cover the electrode wires in the wet blot transfer unit and to soak the gel, membrane and blotting paper. Protein electrophoresis under denaturing conditions (SDS-PAGE) involves separating proteins based on their size. By treating the sample under denaturing and reducing conditions with sodium dodecyl sulfate (SDS), proteins unfold and become coated with SDS detergent molecules, thereby acquiring a high net negative charge that is proportional to the length of their polypeptide chain. During electrophoresis, the negatively-charged protein molecules migrate towards the positive electrode. |
| Size | n/a |
| Concentration | n/a |
| Applications | n/a |
| Other Names | n/a |
| Gene, Accession, CAS # | n/a |
| Catalog # | A12-9123-10 |
| Price | |
| Order / More Info | Tris-Glycine Buffer Powder (pH 8.3) (5 L) (10 pouches) from ANIARA |
| Product Specific References | n/a |
| 產(chǎn)品資料 |
|
ThePorn精品无码,公车上强行被灌满脓液h视频,91精品久久久久
|
国产97视频在线,91麻豆国产精品,全国最嫩91
|
久久久国产无码精品,做爰丰满少妇1314,黄视频免费
|
国产淫乱视频,一边做边爱一边吃奶,操鸡吧
|
国产欧美精品AAAAAA片,丰满放荡岳乱妇91ww,九色|PORNY|蝌蚪视频
|
97香蕉久久国产超碰青草国产区,城中村毛片,成人福利免费视频
|
国产久久在线,欧美日韩美女视频,91资源在线观看
|
99精国产麻豆久久婷婷,91免费黄视频,免费精品视频
|
中文天堂在线观看,日本少妇激三级做爰在线,亚洲视频在线观看视频
|
特污兔成人无码免费,亚洲w码欧洲s码免费,亚洲综合一页
|
|