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產(chǎn)品名稱

U-937 Cell Nuclear Extract from ROCKLAND IMMUNOCHEMICALS INC.

產(chǎn)品貨號

W09-001-GL2

產(chǎn)品價格

現(xiàn)貨詢價，電話：010-67529703

產(chǎn)品規(guī)格

200 μg

產(chǎn)品品牌

ROCKLAND IMMUNOCHEMICALS INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	U-937 Cell Nuclear Extract
Description	U-937 cells were grown in RPMI-1640 medium supplemented with 10% fetal bovine serum. Cells were washed with PBS and then incubated on ice in modified RIPA buffer, containing 150 mM sodium chloride, 50 mM Tris HCl, pH 7.4, 1 mM EDTA, 1.0% NP-40, 0.5% sodium deoxycholic acid , 0.1% SDS and 0.01% (w/v) sodium azide to lyse the cells. Protein integrity was ensured using a cocktail of protease inhibitors with broad specificity for the inhibition of aspartic, cysteine, and serine proteases as well as aminopeptidases (0.1 mM AEBSF HCl, 0.08 μM Aprotinin, 5 μM Bestatin, 1.5 μM E-64, 2 μM Leupeptin Hemisulfate, 1 μM Pepstatin A). Phosphatase inhibitors 1 mM NaF and 1 mM Na3VO4 were also added. Cell debris was removed by centrifugation. Protein concentration was determined by a modified Lowry assay using a commercially available kit. Protein concentration was adjusted to 4 mg/ml in RIPA buffer containing protease and phosphatase inhibitors.
Size	200 μg
Concentration	4.0 mg/mL
Applications	Chip, Immunoprecipitation, Western Blot. Multi-purpose U-937 nuclear extracts are especially prepared as positive control for multiple assays including western blot, immunoprecipitation (IP), capture ELISA or other assays requiring native protein sample. For separation by SDS-PAGE and subsequent western blot analysis, lysates should be diluted by user to desired concentration in SDS-PAGE buffer with 2-mercaptoethanol or dithiothreitol as the reducing agent and heated to 95° C for 5 minutes. Sample is ready for use in immunoprecipitation and ELISA experiments, conditions should be optimized by the user. Rockland recommends its Trueblot IP reagents for immunoprecipitation experiments.
Other Names	U-937 lysate nuclear extract, cell lysate, U937 Nuclear lysate
Gene, Accession, CAS #	n/a
Catalog #	W09-001-GL2
Price
Order / More Info	U-937 Cell Nuclear Extract from ROCKLAND IMMUNOCHEMICALS INC.
Product Specific References	n/a
產(chǎn)品資料

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