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產(chǎn)品名稱(chēng)

Anti-SLA (Soluble Liver Antigens) ELISA from EAGLE BIOSCIENCES INC.

產(chǎn)品貨號(hào)

SLA31-K01

產(chǎn)品價(jià)格

現(xiàn)貨詢(xún)價(jià)，電話(huà)：010-67529703

產(chǎn)品規(guī)格

1 x 96 well

產(chǎn)品品牌

EAGLE BIOSCIENCES INC.

產(chǎn)品概述

產(chǎn)品詳情

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Product Name	Anti-SLA (Soluble Liver Antigens) ELISA
Description	The Eagle Biosciences Anti-SLA ELISA Assay Kit is an enzyme immunoassay for the quantitative determination of IgG antibodies to SLA. The antibodies of the calibrators, controls, and diluted patient samples react with SLA immobilized on the solid phase of microtiter plates. The use of human recombinant SLA guarantees the specific binding of SLA antibodies of the specimen under investigation. Following an incubation period of 30 min at room temperature (RT), unbound serum components are removed by a wash step. The bound IgG antibodies react specifically with anti-human-IgG conjugated to horseradish peroxidase (HRP) within the incubation period of 30 min at RT. Excessive conjugate is separated from the solid-phase immune complexes by the following wash step. HRP converts the colorless substrate solution of 3,3',5,5'-tetramethyl benzidine (TMB) added into a blue product. The enzyme reaction is stopped by dispensing an acidic solution (HCl) into the wells after 30 min at RT turning the solution from blue to yellow. The optical density (OD) of the solution at 450 nm is directly proportional to the amount of specific antibodies bound. The standard curve is established by plotting the antibody concentrations of the calibrators (x-axis) and their corresponding OD values (y-axis) measured. The concentration of antibodies of the specimen is directly read off the standard curve. Sample Types: serum, plasma. Range: 3 -300 U/ml. Sensitivity: 3 U/ml. Time: 2 hours ? The group of primary autoimmune liver disease (PAL) comprises AIH, primary biliary cirrhosis (PBC) and primary sclerosing cholangitis (PSC). The clinical picture of PAL is in most cases not different from other chronic liver diseases. About 15% of all cases with chronic liver diseases show an autoimmune pathogenesis. Therefore, after exclusion of infectious aetiology especially by viruses, the determination of different autoantibodies is recommended. Patients suffering from AIH show a variety of autoantibodies. Due to the appearance of different antibody specificities classification of AIH into different subgroups is discussed. Type I is characterized by the occurrence of antinuclear antibodies (ANA) and antibodies to smooth muscles (ASMA). ASMA recognize Antigensic structure formed by polymeric f-Actin. For type II a high prevalence of antibodies to liver and kidney microsomal Antigenss (LKM) has been described. LKM1 antibodies recognize epitopes of cytochrome P450 IID6, a 50 kDa cyto plasmic protein found in hepatocytes and proximal tubular kidney cells. LC1 antibodies are specific for type II hepatitis, too. The respective Antigens is formiminotransferase / cyclodeaminase located in the cytosol of liver cells. Patients with type III autoimmune hepatitis exhibit antibodies to the soluble liver Antigens (SLA) and to the liver pancreas Antigens (LP). It has been shown that both antibody species recognise the same Antigens and thus are identical. The yet unknown Antigens is suggested to be an UGA suppressor tRNA-associated protein. However, type III AIH is not yet fully accepted as independent subgroup for autoimmune hepatitis by the ``International Hepatitis Group''. SLA is found in up to 20% of AIH cases and very often the only autoantibody associated with AIH to be detected.
Size	1 x 96 well
Concentration	n/a
Applications	RUO
Other Names	SLA31-K01 ASSAY EIA ELISA IMMUNOASSAY KIT, Anti SLA Soluble Liver Antigens
Gene, Accession, CAS #	n/a
Catalog #	SLA31-K01
Price
Order / More Info	Anti-SLA (Soluble Liver Antigens) ELISA from EAGLE BIOSCIENCES INC.
Product Specific References	n/a
產(chǎn)品資料

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