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         Home >Products> Reagents >Cell Biology>  Enzymatic Chromatrap® Standard Pro-G spin column shearing kit 
        


 
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      Enzymatic Chromatrap® Standard Pro-G spin column shearing kit
      
        
					
        
						
        Enzymatic Chromatrap® Standard Pro-G spin column shearing kit
        

						
                         
        Brand: 
        Porvair Sciences Limited
        
						
        Model: 
        Standard Pro-G
        
						
						
						
						
        Updated:
         2/16/2022
        
						
        
							  
							
						
        
           
						
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        • Product Detail
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          Product Description
          

          24 Chromatrap®  Protein-G based spin-columns, buffers and reagents for enzymatic shearing of chromatin, for ChIP assays.
          

          The kit contains sufficient Protein-A based spin-columns, buffers and reagents to perform 24 chromatin immunoprecipitation assays and up to 10 chromatin preparations.
          

          Standard kits contain all of the buffers and filter materails, but do not contain the positive or negative control antibodies.
          

          Kit Contents:
          

          Chromatrap® spin columns 24
          Column conditioning buffer 50mls
          Wash Buffer 1  50mls
          Wash Buffer 2  50mls
          Wash Buffer 3 50mls
          1.3M Glycine  20mls
          Lysis Buffer 10mls
          Digestion Buffer 10mls
          Enzymatic Stop Solution 200µl
          0.1M Elution Solution 3mls
          0.1M NaOH  3mls
          5M NaCl 500µl
          1M NaHCO3 500µl
          Hypotonic Buffer  10mls
          Shearing Cocktail 100µl
          Protease Inhibitor Cocktail (PIC) 100µl
          Proteinase K stop solution 100µl
          Proteinase K  50µl
          1.5ml Collection tube 24
            
        
								
          
								 Porvair Sciences, specialists in the manufacture of microplate products, serve Life Sciences, Biotechnology, R&D and Molecular Biology with microplate solutions for all applications, from sample preparation to high throughput screening via our global distributor network.
          

          
Our range includes vacuum manifolds, sealers, evaporators and microtiter plates in all popular styles; deep well and shallow well storage plates, assay plates, luciferase reporter gene plates and liquid handling reagent reservoirs. We also provide custom microplate products for life science research.
          

          
Our vacuum manifolds, essential for 96-well SPE sample preparation, are designed to work with most popular filter plates, including Waters, Millipore, Qiagen, Whatman, GE Healthcare, Varian, Biotage and, of course, Porvair.
          

          
For further information, including custom built microplates, reagent reservoirs, vacuum manifold design or microplate handling equipment, please contact us: Tel: +44 1978 666240; Email: int.salesporvair-sciences.com.
								
        
							
        
						
        
				
						

            
          
        
            
            
            
            
						
        
							
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